2010-12-15, 01:24 PM
Sorry wasn't able to respond immediately. Stereo I'm not sure whether alkylating agents and topoisomerase inhibitors won't affect native DNA that is present in chromatin form. As they target chemical features of DNA, the conformation of this DNA may not affect chemical binding and the consequent inhibition of these cells resulting in death.
According to the New England Journal of Medicine article, he was on Highly Active Antiretroviral Therapy (HAART) before implantation. Focusing solely on the pre-implantation phase, the presence of HIV1-RNA barely showed up when they attempted to scan for it using Northern Blot or a similar category of tests used to assay for the RNA band. When they took him off HAART then the HIV1-RNA picked up again. Therefore they decided to keep him on it and once again the HIV1-RNA disappeared. The implant took place when HIV1-RNA levels were extremely low, therefore indicating that the HIV machinery used to reproduce (i.e. reverse transcriptase) was not actively present when the mutant red blood cells were given to the patient.
OB3LISK Wrote:The article scammed me then F4. That guy is pretty lucky they chose to use him then. Pain is nothing compared to life.
According to the New England Journal of Medicine article, he was on Highly Active Antiretroviral Therapy (HAART) before implantation. Focusing solely on the pre-implantation phase, the presence of HIV1-RNA barely showed up when they attempted to scan for it using Northern Blot or a similar category of tests used to assay for the RNA band. When they took him off HAART then the HIV1-RNA picked up again. Therefore they decided to keep him on it and once again the HIV1-RNA disappeared. The implant took place when HIV1-RNA levels were extremely low, therefore indicating that the HIV machinery used to reproduce (i.e. reverse transcriptase) was not actively present when the mutant red blood cells were given to the patient.